Search Results
Search for other papers by Mario Monaco in
Google Scholar
PubMed
Search for other papers by Gennaro Chiappetta in
Google Scholar
PubMed
Search for other papers by Concetta Aiello in
Google Scholar
PubMed
Search for other papers by Antonella Federico in
Google Scholar
PubMed
Search for other papers by Romina Sepe in
Google Scholar
PubMed
Search for other papers by Daniela Russo in
Google Scholar
PubMed
Instituto Nacional de Câncer - INCA, Rio de Janeiro, Brazil
Search for other papers by Alfredo Fusco in
Google Scholar
PubMed
Search for other papers by Pierlorenzo Pallante in
Google Scholar
PubMed
Background: Previous analysis of CBX7 expression in a large number of thyroid adenoma and carcinoma samples revealed a progressive reduction of CBX7 levels that was well related with the malignant grade of thyroid neoplasias. Hürthle cell tumors are unusual thyroid neoplasms characterized by the presence of particular cells called oncocytes. Objectives: In order to develop new tools for a more accurate diagnosis of Hürthle cell tumors of the thyroid, we evaluated CBX7 protein levels to verify the possible presence of an expression signature. Methods: CBX7 expression was evaluated by immunohistochemistry in a panel of thyroid tissue sections including normal thyroids, goiters, follicular adenomas and oncocytic lesions. Results: CBX7 expression was low or null in 68% of Hürthle adenomas, whereas it was comparable to normal thyroid tissue in Hürthle hyperplasias and follicular adenomas. Conclusions: Reduced expression of CBX7 suggests a more aggressive identity of Hürthle adenomas with respect to non-Hürthle ones.
Search for other papers by Vincenza Leone in
Google Scholar
PubMed
Search for other papers by Concetta Langella in
Google Scholar
PubMed
Search for other papers by Francesco Esposito in
Google Scholar
PubMed
Search for other papers by Marco De Martino in
Google Scholar
PubMed
Search for other papers by Myriam Decaussin-Petrucci in
Google Scholar
PubMed
Search for other papers by Gennaro Chiappetta in
Google Scholar
PubMed
Search for other papers by Antonio Bianco in
Google Scholar
PubMed
Instituto Nacional de Cancer (INCA), Rio de Janeiro, Brazil
Search for other papers by Alfredo Fusco in
Google Scholar
PubMed
We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR-130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified CCDC6, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity.