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thyrotoxicosis). In the patients with GD, the median TSH receptor antibody level was 16 U/L (range 9–41 U/L). All patients with AIT had type 2 AIT, confirmed by 123 -iodine thyroid scintigraphy (online suppl. Fig.; www.karger.com/doi/10.1159/000507019) or
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IFB Adiposity Diseases, Leipzig University Medical Centre, Leipzig, Germany
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IFB Adiposity Diseases, Leipzig University Medical Centre, Leipzig, Germany
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Treatment For the induction of thyrotoxicosis, mice received L -thyroxine at a dose of 2 µg/ml diluted in drinking water for 4 weeks. Hypothyroidism was induced in 10-week-old mice by feeding iodine-free chow supplemented with 0.15% PTU (catalog TD 97061
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NOVA Medical School, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal
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NOVA Medical School, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal
Unidade de Investigação em Patobiologia Molecular, Instituto Português de Oncologia de Lisboa, Lisbon, Portugal
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, radioactive iodine; WBS, whole-body scan. She received 100 mCi of radioactive iodine (RAI) with recombinant TSH. The stimulated Tg was 7.5 ng/mL and the post-RAI whole-body scan showed a moderate cervical residual uptake (as presented in Fig. 1
Center of Brain, Behavior and Metabolism (CBBM)/Medizinische Klinik I, University of Lübeck, Lübeck, Germany
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Center of Brain, Behavior and Metabolism (CBBM)/Medizinische Klinik I, University of Lübeck, Lübeck, Germany
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Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
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derivatives 3-iodothyronamine (3-T 1 AM) and the iodine-free thyronamine (T 0 AM) have been detected in vivo, e.g., in the brain, heart, liver, and blood of male C57BL/6J mice [ 1 ]. Nevertheless, the effects of T 0 AM are much less studied compared to 3-T 1
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within 4 weeks of their deliveries and cord FT4 level of their infants at the time of delivery were available. Those who had multiple pregnancies, fetal goiter detected by ultrasonography, iodine or glucocorticoid treatment, and change in ATD after the
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Background: Thyroglobulin (Tg) is a biomarker of iodine status. Newborn Tg is a more sensitive marker than neonatal TSH in detecting variations in iodine intake. This study aims to validate a Tg enzyme-linked immunosorbent assay (ELISA) for Tg determination on dried blood spots (DBS) in newborns. This study also set out to assess the stability of Tg and the influence of newborns’ hematocrit on Tg determination.
Methods: A commercially available ELISA Tg assay was adapted for use on DBS. DBS-Tg in cord blood were measured in 209 newborns delivered from healthy euthyroid pregnant women. Sensitivity, linearity, repeatability, and intermediate fidelity were determined using the appropriate standards and quality control materials.
Results: The limit of detection (LoD) of the DBS-Tg assay was 2.4 µg/L, and the limit of quantification (LoQ) was 5.8 µg/L. Repeatability and intermediate fidelity were 7.7-8.3% and 11.0-11.2%, respectively. The median cord plasma Tg and DBS-Tg values in newborns were not significantly different, 30.2 (21.3-44.4) µg/L and 31.6 (19.3-48.7) µg/L (p=0.48) with the ELISA respectively, and 76.5 (40.0-101.5) µg/L with the Elecsys assay with an R=0.88. DBS-Tg concentrations decrease with increasing hematocrit values (p<0.05). DBS-Tg values were stable at a concentration of 25 µg/L for 12 months at -20ºC and 4ºC.
Conclusion: This DBS-Tg assay demonstrated good analytical performances over a wide range of Tg concentrations, suggesting it is well suited to detecting variations in Tg concentrations. Studies comparing populations with different prevalence of anemia should consider the effect of hematocrit on DBS-Tg determination. The availability of a DBS-Tg assay for newborns makes it possible to integrate iodine status monitoring with newborn screening for inherited metabolic diseases.
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Institut National de la Recherche Médicale, UMR U895, University Nice-Sophia-Antipolis, Nice, France
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Dear Editor, We read with interest the comments and suggestions of Dinc et al. [ 1 ] regarding our paper [ 2 ]. The authors state that an iodine supplementation of 150 μg/day was not adequate in women included in our study. However, this
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Dear Sir, The word ‘cretin' was initially used for mentally retarded, deaf-mute and usually goitrous individuals born and living in areas of very severe iodine deficiency [ 1 ]. It is believed to have originated from ‘Pauvre Chrétienne' (a
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WHO. Nationally, he was member of a committee of the Gezondheidsraad (Health Council) advising on iodine supply, radioactive iodine prophylaxis, and iodine prophylaxis in nuclear accidents. His tutorship of students, residents, and colleagues was of
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Granada. He completed his doctoral thesis on the administration of iodized salt in the iodine-deficient region of Alpujarras under the mentorship of Prof. Ortiz de Landazuri. From then on, his scientific career has been inseparable from that of his wife