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Eijun Nishihara Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Yoshitaka Hobo ASKA Pharmamedical Co., Ltd. Fujisawa, Japan

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Akira Miyauchi Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Yasuhiro Ito Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Miyoko Higuchi Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Mitsuyoshi Hirokawa Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Mitsuru Ito Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Shuji Fukata Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Mitsushige Nishikawa Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Takashi Akamizu Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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-tandem mass spectrometry (LC-MS/MS) for Tg quantification has emerged as an alternative method that may not be influenced by TgAb. This method is based on specific peptide quantitation following tryptic digestion of all proteins, including TgAb and

Article Type:
Research Article
Collections:
Thyroid function tests
DOI:
https://doi.org/10.1530/ETJ-21-0041
Volume/Issue:
Volume 11: Issue 1
Open access
Daniel Rathmann Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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Eddy Rijntjes Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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Julika Lietzow Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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Josef Köhrle Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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if the full spectrum of the TH and TAM family is of analytical interest. Technical advances in HPLC liquid chromatography and its subsequent combination with tandem mass spectrometry (LC-MS/MS) provided a highly sensitive method to measure part - most

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000430840
Volume/Issue:
Volume 4: Issue 1_Suppl
Free access
Heleen I Jansen Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands

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Antonius E van Herwaarden Radboud University Medical Center, Department of Laboratory Medicine, Nijmegen, The Netherlands

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Henk J Huijgen Department of Clinical Chemistry, Red Cross Hospital, Beverwijk, The Netherlands

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Rebecca C Painter Department of Obstetrics and Gynaecology, Amsterdam UMC Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands

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Jacquelien J Hillebrand Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands

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Anita Boelen Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands

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Annemieke C Heijboer Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands

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conventional reference measurement procedure of fT4 using liquid chromatography tandem-mass spectrometry (LC-MS/MS), preceded by an equilibrium dialysis method, is aimed to minimally disturb the physiological equilibrium between fT4 and bound T4 in serum to

Article Type:
Research Article
Collections:
Thyroid and reproduction
DOI:
https://doi.org/10.1530/ETJ-22-0145
Volume/Issue:
Volume 11: Issue 6
Open access
Bernadette L Dekker Internal Medicine, Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Anouk N A van der Horst-Schrivers Internal Medicine, Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands
Department of Emergency Medicine, Maastricht University Medical Center, Maastricht, the Netherlands

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Adrienne H Brouwers Department of Nuclear Medicine and Molecular Imaging, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Christopher M Shuford Laboratory Corporation of America Holdings, Center for Esoteric Testing, Burlington, North California, USA

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Ido P Kema Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Anneke C Muller Kobold Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Thera P Links Internal Medicine, Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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-tandem mass spectrometry (LC-MS/MS), which digests Tg into peptides for analytical measurement, disrupting the interfering TgAbs ( 8 ). However, Tg-LC-MS/MS assays studied to date are hampered by a lack of sensitivity compared to Tg immunoassays ( 1 , 2 , 9

Article Type:
Research Article
Collections:
Thyroid cancer - clinical
DOI:
https://doi.org/10.1530/ETJ-22-0137
Volume/Issue:
Volume 11: Issue 6
Open access
Beatrice Engelmann Interfaculty Institute for Genetics and Functional Genomics, Germany

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Julia Bischof Interfaculty Institute for Genetics and Functional Genomics, Germany

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Anne-Luise Dirk Experimental and Clinical Endocrinology, Med Clinic I, University of Lübeck, Lübeck, Germany

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Nele Friedrich Institute for Clinical Chemistry and Laboratory Medicine, Germany

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Elke Hammer Interfaculty Institute for Genetics and Functional Genomics, Germany

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Thomas Thiele Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany

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Dagmar Führer Clinic for Endocrinology and Metabolic Disorders, University Clinics Essen, Essen, Germany

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Georg Homuth Interfaculty Institute for Genetics and Functional Genomics, Germany

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Georg Brabant Experimental and Clinical Endocrinology, Med Clinic I, University of Lübeck, Lübeck, Germany

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Uwe Völker Interfaculty Institute for Genetics and Functional Genomics, Germany

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plasma samples was performed in single LC-MS/MS runs. In total, 2,374 unique peptides representing 497 human proteins were identified. Only proteins identified in more than 60% of all samples (n = 439) were considered for quantification. Among those

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000381769
Volume/Issue:
Volume 4: Issue 1_Suppl
Free access
Lisbeth Harder Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Center of Brain, Behavior and Metabolism (CBBM)/Medizinische Klinik I, University of Lübeck, Lübeck, Germany

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Nancy Schanze Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Assel Sarsenbayeva Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Franziska Kugel Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Josef Köhrle Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Lutz Schomburg Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Jens Mittag Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Center of Brain, Behavior and Metabolism (CBBM)/Medizinische Klinik I, University of Lübeck, Lübeck, Germany

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Carolin S. Hoefig Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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TAM Analysis in Liver Hepatic THs and TAMs were extracted from 80 mg of mouse liver as previously described [ 2 , 13 ] followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis as published [ 14 ]. A brief description of the

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000481856
Volume/Issue:
Volume 7: Issue 1
Free access
Irene Campi Division of Endocrine and Metabolic Diseases and Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy

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Maura Agostini Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom

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Federica Marelli Division of Endocrine and Metabolic Diseases and Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy

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Tiziana de Filippis Division of Endocrine and Metabolic Diseases and Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy

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Beatriz Romartinez-Alonso Department of Molecular and Cell Biology, Leicester Institute of Structural and Chemical Biology, University of Leicester, Leicester, United Kingdom

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Odelia Rajanayagam Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom

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Giuditta Rurale Division of Endocrine and Metabolic Diseases and Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy

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Ilaria Gentile Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy

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Federica Spagnolo Unit of Endocrinology, University Hospital “G. Martino”, Messina, Italy

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Massimiliano Andreasi Laboratorio Analisi Cliniche, Centro di Ricerche e Tecnologie Biomediche, IRCCS Istituto Auxologico Italiano, Cusano Milanino, Italy

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Francesco Ferraù Unit of Endocrinology, University Hospital “G. Martino”, Messina, Italy
Department of Human Pathology of Adulthood and Childhood, University of Messina, Messina, Italy

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Salvatore Cannavò Unit of Endocrinology, University Hospital “G. Martino”, Messina, Italy
Department of Human Pathology of Adulthood and Childhood, University of Messina, Messina, Italy

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Laura Fugazzola Division of Endocrine and Metabolic Diseases and Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy
Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy

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Krishna V. Chatterjee Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom

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Luca Persani Division of Endocrine and Metabolic Diseases and Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano, Istituto Di Ricovero e Cura a Carattere Scientifico (IRCCS), Milan, Italy
Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy

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better consistency may be found by ultrafiltration LC-MS/MS, which is less influenced by the transport proteins [ 16 ]. Interestingly, in this family there was a large variability in the clinical manifestations of RTHβ, being the proband more severely

Article Type:
Case Report
DOI:
https://doi.org/10.1159/000519748
Volume/Issue:
Volume 10: Issue 6
Free access
Dagmar Führer Department of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, Essen, Germany

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Klaudia Brix Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany

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Heike Biebermann Institut für Experimentelle Pädiatrische Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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analysis of TH metabolites in cell culture samples using LC-MS/MS. Their approach is a prerequisite for comprehensive analysis of TH and TH derivatives in more complex biological samples such as blood or tissues of humans and/or animal models. In addition

Article Type:
Editorial
DOI:
https://doi.org/10.1159/000431318
Volume/Issue:
Volume 4: Issue 1_Suppl
Free access
Luca Persani Department of Endocrine and Metabolic Diseases, IRCCS Istituto Auxologico Italiano, Milano, Italy
Department of Medical Biotechnology and Translational Medicine, University of Milan, Milano, Italy

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Patrice Rodien Service d’Endocrinologie-Diabétologie-Nutrition, Centre de référence des maladies rares de la Thyroïde et des récepteurs hormonaux, CHU d’Angers, Angers, France.

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Carla Moran Institute of Metabolic Science, University of Cambridge, Cambridge, UK
Endocrine Section, Beacon Hospital, Dublin, Ireland.
School of Medicine, University College Dublin, Ireland
Endocrinology Department, St Vincent’s University Hospital, Dublin, Ireland

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W Edward Visser Department of Internal Medicine and Rotterdam Thyroid Center, Erasmus University Medical Center, Rotterdam, The Netherlands

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Stefan Groeneweg Department of Internal Medicine and Rotterdam Thyroid Center, Erasmus University Medical Center, Rotterdam, The Netherlands

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Robin Peeters Department of Internal Medicine and Rotterdam Thyroid Center, Erasmus University Medical Center, Rotterdam, The Netherlands

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Samuel Refetoff Departments of Medicine and Paediatrics and Committee on Genetics, The University of Chicago, Chicago, Illinois, USA

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Mark Gurnell Institute of Metabolic Science, University of Cambridge, Cambridge, UK

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Paolo Beck-Peccoz Department of Medical Biotechnology and Translational Medicine, University of Milan, Milano, Italy

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Krishna Chatterjee Institute of Metabolic Science, University of Cambridge, Cambridge, UK

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administering TRIAC twice or thrice daily. The dosage of TRIAC is titrated to control hyperthyroid symptoms and signs and lower circulating free T4 concentrations (TRIAC cross-reacts in immunoassays but not LC-MS/MS assays of T3) ( Recommendation: S; Quality of

Article Type:
Other
Collections:
Thyroid hormone action
DOI:
https://doi.org/10.1530/ETJ-24-0125
Volume/Issue:
Volume 13: Issue 4
Open access
Markus Eszlinger Department of Oncology and Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Heritage Medical Research Building, Calgary, Alberta, Canada, and Institute of Pathology, University Hospital Halle, Halle, Germany

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Alexandra Stephenson Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Heritage Medical Research Building, Calgary, Alberta, Canada

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Shideh Mirhadi Program in Cell Biology, Hospital for Sick Children, and Department of Molecular Genetics, University of Toronto, Peter Gilgan Centre for Research and Learning, Toronto, Ontario, Canada

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Konrad Patyra Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, Turku, Finland

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Michael F Moran Program in Cell Biology, Hospital for Sick Children, and Department of Molecular Genetics, University of Toronto, Peter Gilgan Centre for Research and Learning, Toronto, Ontario, Canada

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Moosa Khalil Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada

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Jukka Kero Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, Turku, Finland
Department of Pediatrics, Turku University Hospital, Turku, Finland

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Ralf Paschke Department of Oncology and Arnie Charbonneau Cancer Institute, Cumming School of Medicine, University of Calgary, Heritage Medical Research Building, Calgary, Alberta, Canada, and Institute of Pathology, University Hospital Halle, Halle, Germany
Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Heritage Medical Research Building, Calgary, Alberta, Canada
Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
Department of Medicine, Cumming School of Medicine, University of Calgary, Heritage Medical Research Building, Calgary, Alberta, Canada

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HOZ mouse in >10 reads. Phosphoproteome analysis Preparation of phosphopeptide eluates and analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) using an EASY-nanoLC 1200 system and an Orbitrap Fusion™ Lumos™ Tribrid™ Mass

Article Type:
Research Article
Collections:
Thyroid cancer - basic and translational
DOI:
https://doi.org/10.1530/ETJ-23-0049
Volume/Issue:
Volume 12: Issue 6
Open access