-tandem mass spectrometry (LC-MS/MS), which digests Tg into peptides for analytical measurement, disrupting the interfering TgAbs ( 8 ). However, Tg-LC-MS/MS assays studied to date are hampered by a lack of sensitivity compared to Tg immunoassays ( 1 , 2 , 9
Bernadette L Dekker, Anouk N A van der Horst-Schrivers, Adrienne H Brouwers, Christopher M Shuford, Ido P Kema, Anneke C Muller Kobold, and Thera P Links
Eijun Nishihara, Yoshitaka Hobo, Akira Miyauchi, Yasuhiro Ito, Miyoko Higuchi, Mitsuyoshi Hirokawa, Mitsuru Ito, Shuji Fukata, Mitsushige Nishikawa, and Takashi Akamizu
immunocapture of Tg-specific peptides ( 4 ). Among various Tg peptides following cleavage at lysine or arginine with trypsin, two specific peptides, VIFDANAPVAVR and FSPDDSAGASALLR, have been reported to be reliable targets for Tg-LC-MS/MS ( 5 , 6 ). Although