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Department of Emergency Medicine, Maastricht University Medical Center, Maastricht, the Netherlands
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-tandem mass spectrometry (LC-MS/MS), which digests Tg into peptides for analytical measurement, disrupting the interfering TgAbs ( 8 ). However, Tg-LC-MS/MS assays studied to date are hampered by a lack of sensitivity compared to Tg immunoassays ( 1 , 2 , 9
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immunocapture of Tg-specific peptides ( 4 ). Among various Tg peptides following cleavage at lysine or arginine with trypsin, two specific peptides, VIFDANAPVAVR and FSPDDSAGASALLR, have been reported to be reliable targets for Tg-LC-MS/MS ( 5 , 6 ). Although