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Institut National de la Recherche Médicale, UMR U895, University Nice-Sophia-Antipolis, Nice, France
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Institut National de la Recherche Médicale, UMR U895, University Nice-Sophia-Antipolis, Nice, France
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level [ 1 , 2 ], serum thyroglobulin (Tg) has been proposed as a marker of ID [ 3 ], including in newborns [ 4 , 5 ]. Maternal iodine supplementation has been shown to improve maternal and cord blood (CB) thyroid tests in areas of moderate and severe ID
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persisted, dyshormongenesis was suspected and an umbilical cord blood sample was drawn during GW 23. Analysis showed fetal hypothyroidism with TSH >100 mU/l (the sample was not further diluted; reference value 6.8 ± 2.9, mean ± SD [ 11 ]) and fT 4 3.8 pmol
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Background: Thyroglobulin (Tg) is a biomarker of iodine status. Newborn Tg is a more sensitive marker than neonatal TSH in detecting variations in iodine intake. This study aims to validate a Tg enzyme-linked immunosorbent assay (ELISA) for Tg determination on dried blood spots (DBS) in newborns. This study also set out to assess the stability of Tg and the influence of newborns’ hematocrit on Tg determination.
Methods: A commercially available ELISA Tg assay was adapted for use on DBS. DBS-Tg in cord blood were measured in 209 newborns delivered from healthy euthyroid pregnant women. Sensitivity, linearity, repeatability, and intermediate fidelity were determined using the appropriate standards and quality control materials.
Results: The limit of detection (LoD) of the DBS-Tg assay was 2.4 µg/L, and the limit of quantification (LoQ) was 5.8 µg/L. Repeatability and intermediate fidelity were 7.7-8.3% and 11.0-11.2%, respectively. The median cord plasma Tg and DBS-Tg values in newborns were not significantly different, 30.2 (21.3-44.4) µg/L and 31.6 (19.3-48.7) µg/L (p=0.48) with the ELISA respectively, and 76.5 (40.0-101.5) µg/L with the Elecsys assay with an R=0.88. DBS-Tg concentrations decrease with increasing hematocrit values (p<0.05). DBS-Tg values were stable at a concentration of 25 µg/L for 12 months at -20ºC and 4ºC.
Conclusion: This DBS-Tg assay demonstrated good analytical performances over a wide range of Tg concentrations, suggesting it is well suited to detecting variations in Tg concentrations. Studies comparing populations with different prevalence of anemia should consider the effect of hematocrit on DBS-Tg determination. The availability of a DBS-Tg assay for newborns makes it possible to integrate iodine status monitoring with newborn screening for inherited metabolic diseases.
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-dependent influence of ATDs on the difference in FT4 levels between maternal and cord blood in patients with Graves’ disease (GD) [ 2 , 9 ]. In this connection, future research proposed by the ATA guidelines task force includes a trial assessing the optimal target FT
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Dear Editor, We read the article ‘Relative impact of iodine supplementation and maternal smoking on cord blood thyroglobulin in pregnant women with normal thyroid function' by Hiéronimus et al. [ 1 ] with great interest. The authors
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Institut National de la Recherche Médicale, UMR U895, University Nice-Sophia-Antipolis, Nice, France
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iodine supplementation and maternal smoking on cord blood thyroglobulin in pregnant women with normal thyroid function. Eur Thyroid J 2012;1:264-273. 10.1159/000342915 3 Stagnaro-Green A, Abalovich M, Alexander E, Azizi F, Mestman J, Negro R
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Gy over a period 30 days. D max (the maximum dose) of the spinal cord and left intervertebral vessel and D mean (the mean dose) of the larynx and hypopharynx were mainly considered. The equivalent dose for a 2-Gy fraction schedule was calculated
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The Rappaport Faculty of Medicine, Technion, Institute of Technology, Haifa, Israel
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recommended that in cases of high maternal TSI (≥3.7 times the upper reference limit), or when results are not available, umbilical cord blood be taken for TSI, TSH and FT4 determinations and the infant be examined for signs of hyperthyroidism ( 2 , 14
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of their blood supply. On retraction of the thyroid lobe medially, the TZ, if present, is identified. The TZ is mobilized by dividing vascular and fibrous tissue at its apex (laterally) and along the superior and inferior borders. The TZ is then
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Introduction Excessively increased levels of white blood cells (WBC), especially neutrophils, typically guide the diagnosis towards severe acute infection, inflammation, or myeloproliferative neoplasms (MPNs). However, in patients with