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Eijun Nishihara Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Yoshitaka Hobo ASKA Pharmamedical Co., Ltd. Fujisawa, Japan

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Akira Miyauchi Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Yasuhiro Ito Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Miyoko Higuchi Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Mitsuyoshi Hirokawa Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Mitsuru Ito Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Shuji Fukata Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Mitsushige Nishikawa Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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Takashi Akamizu Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, Japan

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-tandem mass spectrometry (LC-MS/MS) for Tg quantification has emerged as an alternative method that may not be influenced by TgAb. This method is based on specific peptide quantitation following tryptic digestion of all proteins, including TgAb and

Article Type:
Research Article
Collections:
Thyroid function tests
DOI:
https://doi.org/10.1530/ETJ-21-0041
Volume/Issue:
Volume 11: Issue 1
Open access
Daniel Rathmann Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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Eddy Rijntjes Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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Julika Lietzow Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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Josef Köhrle Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

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if the full spectrum of the TH and TAM family is of analytical interest. Technical advances in HPLC liquid chromatography and its subsequent combination with tandem mass spectrometry (LC-MS/MS) provided a highly sensitive method to measure part - most

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000430840
Volume/Issue:
Volume 4: Issue 1_Suppl
Free access
Heleen I Jansen Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands

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Antonius E van Herwaarden Radboud University Medical Center, Department of Laboratory Medicine, Nijmegen, The Netherlands

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Henk J Huijgen Department of Clinical Chemistry, Red Cross Hospital, Beverwijk, The Netherlands

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Rebecca C Painter Department of Obstetrics and Gynaecology, Amsterdam UMC Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands

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Jacquelien J Hillebrand Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands

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Anita Boelen Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands

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Annemieke C Heijboer Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
Amsterdam Gastroenterology, Endocrinology & Metabolism, Amsterdam, The Netherlands
Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands
Amsterdam Reproduction & Development Research Institute, Amsterdam, The Netherlands

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conventional reference measurement procedure of fT4 using liquid chromatography tandem-mass spectrometry (LC-MS/MS), preceded by an equilibrium dialysis method, is aimed to minimally disturb the physiological equilibrium between fT4 and bound T4 in serum to

Article Type:
Research Article
Collections:
Thyroid and reproduction
DOI:
https://doi.org/10.1530/ETJ-22-0145
Volume/Issue:
Volume 11: Issue 6
Open access
Bernadette L Dekker Internal Medicine, Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Anouk N A van der Horst-Schrivers Internal Medicine, Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands
Department of Emergency Medicine, Maastricht University Medical Center, Maastricht, the Netherlands

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Adrienne H Brouwers Department of Nuclear Medicine and Molecular Imaging, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Christopher M Shuford Laboratory Corporation of America Holdings, Center for Esoteric Testing, Burlington, North California, USA

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Ido P Kema Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Anneke C Muller Kobold Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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Thera P Links Internal Medicine, Department of Endocrinology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands

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-tandem mass spectrometry (LC-MS/MS), which digests Tg into peptides for analytical measurement, disrupting the interfering TgAbs ( 8 ). However, Tg-LC-MS/MS assays studied to date are hampered by a lack of sensitivity compared to Tg immunoassays ( 1 , 2 , 9

Article Type:
Research Article
Collections:
Thyroid cancer - clinical
DOI:
https://doi.org/10.1530/ETJ-22-0137
Volume/Issue:
Volume 11: Issue 6
Open access
Beatrice Engelmann Interfaculty Institute for Genetics and Functional Genomics, Germany

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Julia Bischof Interfaculty Institute for Genetics and Functional Genomics, Germany

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Anne-Luise Dirk Experimental and Clinical Endocrinology, Med Clinic I, University of Lübeck, Lübeck, Germany

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Nele Friedrich Institute for Clinical Chemistry and Laboratory Medicine, Germany

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Elke Hammer Interfaculty Institute for Genetics and Functional Genomics, Germany

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Thomas Thiele Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany

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Dagmar Führer Clinic for Endocrinology and Metabolic Disorders, University Clinics Essen, Essen, Germany

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Georg Homuth Interfaculty Institute for Genetics and Functional Genomics, Germany

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Georg Brabant Experimental and Clinical Endocrinology, Med Clinic I, University of Lübeck, Lübeck, Germany

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Uwe Völker Interfaculty Institute for Genetics and Functional Genomics, Germany

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plasma samples was performed in single LC-MS/MS runs. In total, 2,374 unique peptides representing 497 human proteins were identified. Only proteins identified in more than 60% of all samples (n = 439) were considered for quantification. Among those

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000381769
Volume/Issue:
Volume 4: Issue 1_Suppl
Free access
Riccardo Donzelli Departments of Pathology, University of Pisa

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Daria Colligiani Departments of Clinical and Experimental Medicine, University of Pisa

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Claudia Kusmic Departments of CNR Institute of Clinical Physiology, Pisa, Italy

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Martina Sabatini Departments of Pathology, University of Pisa

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Leonardo Lorenzini Departments of Pathology, University of Pisa

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Alice Accorroni Departments of Pathology, University of Pisa
Departments of Scuola Superiore Sant'Anna, Pisa, Italy

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Monica Nannipieri Departments of Clinical and Experimental Medicine, University of Pisa

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Alessandro Saba Departments of Pathology, University of Pisa

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Giorgio Iervasi Departments of CNR Institute of Clinical Physiology, Pisa, Italy

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Riccardo Zucchi Departments of Pathology, University of Pisa

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-Aldrich. Solvents for HPLC-MS/MS measurements were LC-MS grade, and the other chemicals were HPLC or reagent grade. Experimental Procedures Principles of laboratory animal care were followed and the study was performed in accordance with the European

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000443523
Volume/Issue:
Volume 5: Issue 1
Free access
Noriyoshi Takahashi Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Haruo Matsushita Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Rei Umezawa Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Takaya Yamamoto Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Yojiro Ishikawa Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Yu Katagiri Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Shun Tasaka Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Kazuya Takeda Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Katsuya Fukui Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Noriyuki Kadoya Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Kengo Ito Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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Keiichi Jingu Department of Radiation Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan

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OS. Dandekar et al. [ 8 ] reported that 31 patients received a protocol in which the total planned dose was 60.8 Gy in twice-daily fractions of 1.8 and 2 Gy and that local control (LC) was achieved in 27 patients, but the median OS was only 70 days

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000493315
Volume/Issue:
Volume 8: Issue 1
Free access
Camille Buffet Department of Thyroid Pathologies and Endocrine Tumors, AP-HP, Pitié-Salpêtrière Hospital, Groupe de Recherche Clinique n°16 Tumeurs Thyroïdiennes, Sorbonne Université, Paris, France
UMR9019, Genome Integrity and Cancers, CNRS, Villejuif, France

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Sophie Leboulleux Department of Nuclear Medicine and Endocrine Oncology, Gustave Roussy Institut, Villejuif, France

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Françoise Kraeber-Bodéré Nuclear Medicine Department, Université de Nantes, CHU de Nantes, CNRS, Inserm, CRCINA, Nantes, France
CHU Nantes/ICO, Saint-Herblain, France

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Caroline Bodet-Milin Nuclear Medicine Department, Université de Nantes, CHU de Nantes, CNRS, Inserm, CRCINA, Nantes, France
CHU Nantes/ICO, Saint-Herblain, France

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Laure Cabanes Department of Cardiology, APHP, Cochin Hospital, Paris, France
Université de Paris, Paris, France

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Anthony Dohan Radiology Department, Université de Paris, Paris, France
Department of Radiology, AP-HP, Hôpital Cochin, Paris, France

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Pascal Leprince Department of Thoracic and Cardiovascular Surgery, Sorbonne Université, AP-HP, Pitié-Salpêtrière Hospital, Paris, France

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Martin Schlumberger UMR9019, Genome Integrity and Cancers, CNRS, Villejuif, France
Department of Nuclear Medicine and Endocrine Oncology, Gustave Roussy Institute, Villejuif, France

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Olivier Huillard Université de Paris, Sorbonne Paris Cité, Paris, France
Department of Medical Oncology, AP-HP, Hôpital Cochin, Paris, France

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Lionel Groussin INSERM Unité 1016, CNRS UMR 8104, Institut Cochin, Paris, France
Université de Paris, Paris, France
Department of Endocrinology, AP-HP, Hôpital Cochin, Paris, France

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receive any specific grant. Author Contributions Contribution to the conception of the work: C.B., L.G., S.L., and M.S. Analysis or interpretation of data for the work: L.C., F.K.B., C.B.M., A.D., P.L., and O.H. Drafting the work: C.B., L.G., S

Article Type:
Case Report
DOI:
https://doi.org/10.1159/000517716
Volume/Issue:
Volume 10: Issue 6
Open access
Lisbeth Harder Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Center of Brain, Behavior and Metabolism (CBBM)/Medizinische Klinik I, University of Lübeck, Lübeck, Germany

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Nancy Schanze Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Assel Sarsenbayeva Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Franziska Kugel Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Josef Köhrle Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Lutz Schomburg Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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Jens Mittag Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Center of Brain, Behavior and Metabolism (CBBM)/Medizinische Klinik I, University of Lübeck, Lübeck, Germany

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Carolin S. Hoefig Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Institute for Experimental Endocrinology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany

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TAM Analysis in Liver Hepatic THs and TAMs were extracted from 80 mg of mouse liver as previously described [ 2 , 13 ] followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis as published [ 14 ]. A brief description of the

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000481856
Volume/Issue:
Volume 7: Issue 1
Free access
Linda M. Thienpont Laboratory for Analytical Chemistry, Faculty of Pharmaceutical Sciences, Gent University, Gent

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Katleen Van Uytfanghe Laboratory for Analytical Chemistry, Faculty of Pharmaceutical Sciences, Gent University, Gent

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Sofie Van Houcke Laboratory for Analytical Chemistry, Faculty of Pharmaceutical Sciences, Gent University, Gent

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Barnali Das Biochemistry and Immunology Laboratory, Kokilaben Dhirubhai Ambani Hospital and Medical Research Institute, Mumbai, India

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James D. Faix Stanford University School of Medicine, Palo Alto, Calif., Abbott Park, Ill., USA

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Finlay MacKenzie Birmingham Quality/UK NEQAS, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK

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Frank A. Quinn Medical and Scientific Affairs, Abbott Diagnostics, Abbott Laboratories, Abbott Park, Ill., USA

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Michael Rottmann Roche Diagnostics GmbH, Penzberg, Germany

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Annick Van den Bruel Department of Endocrinology, General Hospital Sint Jan, Bruges, Belgium

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-liquid chromatography-tandem mass spectrometry (ED ID-LC/tandem MS) [ 15 ]. In the case of TSH, for which it is technically unlikely to have an RMP available in the mid- to short term, the C-STFT proposed a statistical alternative for ‘harmonization' rather than

Article Type:
Research Article
DOI:
https://doi.org/10.1159/000358270
Volume/Issue:
Volume 3: Issue 2
Open access