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(such as embolization or chemoembolization) and systemic therapy can be the only therapeutic options ( 2 ). In last decades, transarterial radioembolization (TARE), also known as selective internal radiation therapy (SIRT) ( 6 , 7 ), with microspheres
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Objective: This study examined the effect of Sirtuin 4 (Sirt4), a NAD+-dependent deacetylase, on the proliferation and progression of papillary thyroid carcinoma (PTC).
Methods: Data from The Cancer Genome Atlas (TCGA) were analyzed to identify Sirt4 expression in thyroid cancer. Subsequently, the correlation between Sirt4 expression and clinical characteristics was examined in 205 PTC tissue samples. In vitro assays using three human thyroid cancer cell lines (B-CPAP, TPC-1, and SNU-790) were conducted to assess the effects of regulated Sirt4 expression on cell growth, apoptosis, invasion, and migration. Furthermore, in vivo experiments were performed in a xenograft mouse model.
Results: GEO and TCGA data indicated that Sirt4 expression is lower in thyroid cancer and Sirt4 downregulation is associated with poor overall survival. In our PTC tissues, positive Sirt4 expression was associated with decreased extracapsular extension. In in vitro experiments using three human thyroid cancer cell lines, overexpression of Sirt4 decreased cell survival, clonogenic potential, and invasion and migratory capabilities, as well as inducing apoptosis and increasing reactive oxygen species levels. Sirt4 overexpression upregulated E-cadherin and downregulated N-cadherin, suggesting its potential involvement in the regulation of epithelial–mesenchymal transition. These findings were confirmed in vivo using a xenograft mouse model.
Conclusion: This study provides novel insight into the potential contribution of Sirt4 to regulation of the pathological progression of PTC. The data suggest that Sirt4 plays a tumor-suppressive role in PTC by inhibiting growth, survival, and invasive potential. Future research should investigate the molecular mechanisms underlying these effects of Sirt4.
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involved the regulation of TH target genes [ 30 ]. SIRT1 (the first of 7 different sirtuin enzymes) is a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase that removes the acetyl groups from proteins, such as histones, thereby affecting the
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. Glycemic status in hyperthyroid subjects . Mymensingh Med J . 2004 Jan ; 13 ( 1 ): 71 – 5 . 14747791 1022-4742 3 Suh JH , Sieglaff DH , Zhang A , Xia X , Cvoro A , Winnier GE , et al. SIRT1 is a direct coactivator of thyroid
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Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
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Department of Medicine, College of Medicine and King Saud Medical City, King Saud University, Riyadh, Saudi Arabia
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Department of Biochemistry and Molecular Medicine, College of Medicine, Alfaisal University, Riyadh, Saudi Arabia
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.ejim.2016.03.028 ) 44 Chau MD Gao J Yang Q Wu Z & Gromada J . Fibroblast growth factor 21 regulates energy metabolism by activating the AMPK-SIRT1-PGC-1alpha pathway . Proceedings of the National Academy of Sciences of the United States of
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affected by pharmacological daily doses of 10 and 25 mg/kg 3-T1AM, and SIRT4- and SIRT6-dependent genes responded in the liver after 3-T1AM administration in subchronic protocols using female CD1 mice [188]. Analysis of 3-T1AM concentration in various
