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-tandem mass spectrometry (LC-MS/MS), which digests Tg into peptides for analytical measurement, disrupting the interfering TgAbs ( 8 ). However, Tg-LC-MS/MS assays studied to date are hampered by a lack of sensitivity compared to Tg immunoassays ( 1 , 2 , 9

-tandem mass spectrometry (LC-MS/MS) for Tg quantification has emerged as an alternative method that may not be influenced by TgAb. This method is based on specific peptide quantitation following tryptic digestion of all proteins, including TgAb and

TAM Analysis in Liver Hepatic THs and TAMs were extracted from 80 mg of mouse liver as previously described [ 2 , 13 ] followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis as published [ 14 ]. A brief description of the

.10.042 19114271 24 Anckaert E, Poppe K, Van Uytfanghe K, Schiettecatte J, Foulon W, Thienpont LM: FT4 immunoassays may display a pattern during pregnancy similar to the dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of

explores DuOx regulation by TSH, cAMP and CREB (cAMP response element-binding protein), as these factors also regulate TPO (thyroperoxidase) and Tg (thyroglobulin) expression [ 13 ]. Furthermore, as insulin and insulin-like growth factor 1 (IGF-1) have

role of TKIs in the management of advanced thyroid cancer, several concerns remain to be addressed (Fig. 2 ). Fig. 2 Topics that remain to be elucidated in the treatment of thyroid cancer with TKIs. TKI, tyrosine kinase inhibitor; Tg

better consistency may be found by ultrafiltration LC-MS/MS, which is less influenced by the transport proteins [ 16 ]. Interestingly, in this family there was a large variability in the clinical manifestations of RTHβ, being the proband more severely

analysis of TH metabolites in cell culture samples using LC-MS/MS. Their approach is a prerequisite for comprehensive analysis of TH and TH derivatives in more complex biological samples such as blood or tissues of humans and/or animal models. In addition

significant vs. 125 ng WT-NKX2-1 + 125 ng empty vector. Luciferase Assay The luciferase activity detected in cells transiently transfected with 250 ng of WT- NKX2-1 cDNA is about fourfold higher over baseline, whereas no activation of the TG

: an LC/MS characterization . Anal Biochem . 2006 Feb ; 349 ( 2 ): 197 – 207 . 10.1016/j.ab.2005.11.012 16360109 0003-2697 25 Parekh RB , Dwek RA , Sutton BJ , Fernandes DL , Leung A , Stanworth D , et al. Association of